Tuesday, 25 June 2013

Need to teach PCR?

Need some high quality diagrams to explain polymerase chain reaction (PCR), designing primers, or some combo of both? You have come to the right place! There seemed to be a complete lack of high quality diagrams of primer sequences and the PCR cycle, so I drew some.


The primers. http://en.wikipedia.org/wiki/File:Primers_RevComp.svg



 Melting the template DNA. http://en.wikipedia.org/wiki/File:Primers_RevComp_Melted2.svg


Annealing of the primers. http://en.wikipedia.org/wiki/File:Primers_RevComp_Annealed2.svg


Elongation by DNA polymerase from the primers. http://en.wikipedia.org/wiki/File:Primers_RevComp_Elongation2.svg

These diagrams are all in scalable vector graphics (SVG) format and free for others to use and edit. Inkscape is a great, free, SVG editor, feel free to grab it and modify these images to your heart's content!

Software used:
Inkscape: Vector graphics
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3 comments:

  1. The EGE26 June 2013 at 00:41

    As a Wikipedia editor, thank you so much for all the high-quality images you've contributed. It's a great help - and good inspiration - for those of us with lesser Inkscape skills.

    ReplyDelete
    Replies
    1. Zephyris6 July 2013 at 18:09

      Thanks, it is always great to hear my work is appreciated!

      Delete
  2. grunen11 January 2014 at 13:00

    Thanks a lot, real good work. Svg files allow us to include high quality illustrations into our paper. Inskape rules! After finishing my pHD, I'll try to spend more time to learn this really good software

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